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KMID : 0613820110210111579
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Journal of Life Science
2011 Volume.21 No. 11 p.1579 ~ p.1585
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Inhibition of α-Glucosidase by a Semi-Purified Ethyl Acetate Fraction from Submerged-Liquid Culture of Agaricus blazei Murill
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Jung Kwan-Ju
Moon Yeon-Gyu
Kwon Jung-Min
Ahn Chae-Rin
Kim Jeong-Ok
Ha Yeong-Lae
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Abstract
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Natural anti-diabetic semipurified ethyl acetate fraction was isolated from the submerged-liquid culture of Agaricus blaze Murill (AB) in a medium containing soybean flakes. Hot-water extract of AB (HEAB) was prepared by extraction at 121℃ for 60 min, followed by filtering through a filter presser filled with diatomate. The β-glucan-free HEAB, which was a supernatant fraction from HEAB by precipitation in an 80% ethanol solution, was fractionated into hexane, chloroform, ethyl acetate, and butanol fractions. The inhibition of the α-glucosidase activity by fractions was 59.0, 17.0, 61.6, and 37.9%, respectively, suggesting that ethyl acetate fraction was the most active. A subfraction having a strong α-glucosidase inhibitory activity (80.4%) was isolated from the ethyl acetate fraction. This subfraction contained isoflavones (genistin and daidzin) and their conjugates with sugars as potent inhibiters of α-glucosidase activity. These results suggest that the ethyl acetate fraction or HEAB containing isoflavones and their sugars conjugates could be useful sources for controlling blood sugar levels in humans.
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KEYWORD
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Agaricus blazei Murill (AB), α-glucosidase, isoflavone, isoflavone conjugates with sugars
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